Affinity Maturation

Affinity Maturation

Our Advantages

FCMES-AM™
FCMES-AM™

Full Coverage Mammalian Expression System for Affinity Maturation

No-bias Site Saturation  Mutagenesis in CDR Region
No-bias Site Saturation Mutagenesis in CDR Region

Guarantee at least 5-fold  Affinity Improvement
Guarantee at least 5-fold Affinity Improvement

FCMES-AM™

FCMES-AM™ (Full Coverage Mammalian Expression System for Affinity Maturation) is Biointron's proprietary platform for affnity maturation. For each amino acid in CDR region, it will be mutated to the other 18 amino acids (except Cystein) with equal ratio. The clones will be expressed in mammalian system and ELISA binding assay will be conducted as initial test, followed by SPR or FACS for further affinity measurement.


FC-MES™



Affinity Maturation Service Detail

MilestoneService DescriptionDeliverables

Sequence Design

No-bias Site Saturation Mutagenesis in CDR Region

(all 18 amino acid variants for each amino acid in CDR region )

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Hot Spots

Identification

High Throughput Expression in Mammalian System (FCMES-AM™)

ELISA Detection

ELISA Results

Hot Spots 

Combination

High Throughput Expression in Mammalian System (FCMES-AM™)

ELISA Detection

ELISA Results

Antibody Expression

& Affinity Detection

Antibody Expression & Affinity Purification

Affinity Detection by Biacore Full KD or FACS

Purified Antibody

Plasmid Sequencing Results

Affinity Detection Results


Affinity Maturation Case 1 (VHH)

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Koff of the VHH improves and the affinity increases 6-fold, 23-fold and 19-fold separately.



Affinity Maturation Case 2 (mouse IgG)

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Affinity increases 120-fold, 48-fold and 46-fold separately.