Recombinant antibody technologies are rapidly becoming available and showing considerable clinical success. However, murine monoclonal antibodies may induce immunogenic responses, potentially limiting their application for humans. Humanized antibodies can overcome these problems and are considered to be a promising alternative therapeutic agent.
BioIntron can provide antibody humanization service based on the CDR grafting and back mutation platform.
(comparable to parental antibody / no cost if fail to reach the guaranteed affinity)
(as short as 4-5 weeks to obtain humanized antibody)
(10+years in humanized antibody design / produce 20,000 antibodies)
| Service Step | Service Description | Timeline | Price（USD） | Deliverable |
|---|---|---|---|---|
Design of humanized antibody | ▸Bioinformatics analysis ▸3D structure modeling and identification of back mutations ▸Human germlines selection ▸In silico CDR-grafting ▸Sequence optimization | ~2-3 days | Inquiry | Designed humanized antibody sequences |
| Humanized antibody recombinant production variants 9 to 25 | ▸Codon optimization and gene synthesis ▸Subcloning into expression vector ▸Transient expression ▸Purification ▸QC analysis | 2 weeks | Purified humanized antibody samples for in house test | |
| Affinity Ranking | Affinity Determination with soluble antigen via Biacore 8K | ~1 week | Detailed report |