Human antibody libraries can be made in virtually any display system. Phage display is the most widely used method for de novo discovery of human antibodies.
BioIntron has used its own Phage Display platform to generate a naïve human scFv library. The library is constructed to use multiple “naïve” donors to increase the diversity and capacity. It can help biotech/biopharma to screening their potential antibody drug.
(Multiple rounds panning to obtain high affinity antibodies being in the picomolar range)
(Thousands PBMC sample for library generation / Library Capacity to 10^10 )
(combined solid phase and liquid phase panning methods)
| Service Step | Service Description | Timeline | Deliverable |
|---|---|---|---|
Antigen preparation or Antigen validation | ▸Antigen express by BioIntron in Mammalian system ▸Antigen provided by client | 2-3 weeks | Purified antigen protein (expression by BioIntron) |
| scFv Library Screening with Coated Antigen | ▸Coat well with protein antigen and incubate with phage library ▸ Wash way non-binders ▸Elute binders ▸ELISA to determine specificity of binding（repeat screening for 3-5 rounds） | 4-5 weeks | Biopanning and screening report |
Antibody Characterization | ▸Sequencing for the positive binders ▸Sequencing analysis ▸Transient expression ▸Affinity Ranking by Biocore T200 | 1 week | ▸VH and VL sequence obtain from the scFv library ▸Affinity Ranking report |
4F, G25 building, China Medical City, Taizhou, Jiangsu, China
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