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Antibody Expression

Enhance the efficacy of your antibodies with our powerful platform
CHOK1-Fut8KO expression platform for afucosylated antibodies. Combined with our high-throughput recombinant antibody expression platform and cell culture technology, afucosylated antibodies can be rapidly produced for your research and industrial needs. Afucosylation has been shown to significantly boost antibodydependent cell-mediated cytotoxicity (ADCC), opening up a realm of possibilities for targeted therapies by increasing effectiveness.

Figure 1: Mechanisms of Antibody Action

Antibodies with high purity and low endotoxin levels!

We use advanced gene-editing technology to knock out the fucosyltransferase (Fut8) alleles in the genome of the self-engineered CHOK1BN cells. Fut8 is an enzyme that catalyzes the formation of α-1,6 fucosyl glycosidic linkages. By targeting and eliminating the gene responsible for this enzyme's expression in the CHO cell genome, the cells become incapable of performing normal fucosylation on secreted antibodies. This approach effectively achieves the objective of eliminating fucosylation.1

Fucose-free host cell CHOK1BN-Fut8KO can be used for industrial production after antibody characterization and functional verification. Compared to the unmodified CHOK1BN cell line, the antibody produced by the modified cell line not only achieves afucosylation but also maintains consistency in terms of expression quantity and quality.

Figure 2: FUT8 Gene's Role in Fucosylation Modulation

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1.Ma, M., Fu, Y., Zhou, X., Guan, F., Wang, Y., & Li, X. (2019). Functional roles of fucosylated and O-glycosylated cadherins during carcinogenesis and metastasis. Cellular Signalling, 63, 109365. https://doi.org/10.1016/j.cellsig.2019.109365

2.Pereira, N. A., Chan, K. F., Lin, P. C., & Song, Z. (2018). The "less-is-more" in therapeutic antibodies: Afucosylated anti-cancer antibodies with enhanced antibody-dependent cellular cytotoxicity. mAbs, 10(5), 693–711. https://doi.org/10.1080/19420862.2018.1466767

3.Fischer, S., Handrick, R. and Otte, K. (2015) ‘The art of cho cell engineering: A comprehensive retrospect and future perspectives’, Biotechnology Advances, 33(8), pp. 1878–1896. doi:10.1016/j.biotechadv.2015.10.015.