• Biointron Launches New Referral Program! 25% OFF on Expression Services> Learn More
  • 10% OFF on your next discovery & optimization projects! AbExplorer Rewards> Learn More
  • 40% OFF on Catalog Products! Explore Explore Abinvivo (A Biointron brand)> Learn More
  • RushMab™ - Small Scale Packages for Supernatant and Purified Antibody with Expression Plasmids> Learn More
  • Biointron Introduces CHOK1-FUT8 for Afucosylated Antibody Expression Platform> Learn More

HTP Recombinant Antibody/VHH Production

HTP Recombinant Antibody/VHH Production

As a result of the advances in antibody discovery technologies in hybridoma, display libraries, Single B-cell sorting/sequencing and computational modeling, thousands of mAbs have been generated in antibody discovery campaigns. To rapidly find “best-in-class” antibody therapeutics, it has become essential to develop high throughput (HTP) processes that allow rapid assessment of antibodies for functional and molecular properties.

BioIntron has developed a high throughput (HTP) platform to offer our customers the flexibility to rapidly express a large number of antibodies at various scale in HEK293/CHO cell.

Our Advantages

Fast Turnaround Time
Fast Turnaround Time

(Only 2 weeks from gene synthesis to purified antibody)

Amount Guaranteed
Amount Guaranteed

(up to mg level / no antibody no charge)

Competitive Price
Competitive Price

(starting from $599/rAb including gene synthesis)

HTP Recombinant Antibody/VHH Production Working Flowchart


HTP Recombinant Antibody/VHH Production Working Flowchart

HTP Recombinant Antibody/VHH Production Service Detail

Service StepService DescriptionTimelinePrice(USD)Deliverable

Gene synthesis and cloning

Codon optimization and gene synthesis

Subcloning into an expression vector

Plasmid amplification and preparation

1 weeks

Inquiry

Expression plasmid contain GOI

Purified antibody (SDS-PAGE>95%, endotoxin level <1EU/mg, including SEC-HPLC detection)

CoA report

Transient expression & purification

Transfection of mammalian cells

Transient expression and purification

1 weeks

Case Study: Anti-PD1 Antibody Small Scale Expression

SEC-HPLC Detection Result

Lane M: Marker Lane 1: Non-Reducing Lane 2:Reducing

SEC-HPLC Detection Result

SEC-HPLC Result

Expression result
Yield (mg/L)SDS-PAGE %SEC-HPLC%Endotoxin (EU/mg)Timeline
459>95%99.3%
<1EU/mg14 days