HTP Recombinant Antibody/VHH Production-Biointron

As a result of the advances in antibody discovery technologies in hybridoma, display libraries, Single B-cell sorting/sequencing and computational modeling, thousands of mAbs have been generated in antibody discovery campaigns. To rapidly find “best-in-class” antibody therapeutics, it has become essential to develop high throughput (HTP) processes that allow rapid assessment of antibodies for functional and molecular properties.

BioIntron has developed a high throughput (HTP) platform to offer our customers the flexibility to rapidly express a large number of antibodies at various scale in HEK293/CHO cell.

Our Advantages

Fast Turnaround Time

(Only 2 weeks from gene synthesis to purified antibody)

Amount Guaranteed

(up to mg level / no antibody no charge)

Competitive Price

(starting from $599/rAb including gene synthesis)

HTP Recombinant Antibody/VHH Production Working Flowchart

HTP Recombinant Antibody/VHH Production Service Detail

Service Step	Service Description	Timeline	Price（USD）	Deliverable
Gene synthesis and cloning	▸Codon optimization and gene synthesis ▸Subcloning into an expression vector ▸Plasmid amplification and preparation	1 weeks	Inquiry	▸Expression plasmid contain GOI ▸Purified antibody (SDS-PAGE>95%, endotoxin level <1EU/mg, including SEC-HPLC detection) ▸CoA report
Transient expression & purification	▸Transfection of mammalian cells ▸Transient expression and purification	1 weeks

Service Step

Service Description

Timeline

Price（USD）

Deliverable

Gene synthesis and cloning

▸Codon optimization and gene synthesis

▸Subcloning into an expression vector

▸Plasmid amplification and preparation

1 weeks

Inquiry

▸Expression plasmid contain GOI

▸Purified antibody (SDS-PAGE>95%, endotoxin level <1EU/mg, including SEC-HPLC detection)

▸CoA report

Transient expression & purification

▸Transfection of mammalian cells

▸Transient expression and purification

1 weeks

Case Study: Anti-PD1 Antibody Small Scale Expression

Lane M: Marker Lane 1: Non-Reducing Lane 2:Reducing		SEC-HPLC Result
Expression result
Yield (mg/L)	SDS-PAGE %	SEC-HPLC%	Endotoxin (EU/mg)	Timeline
459	>95%	99.3%	<1EU/mg	14 days