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HTP Recombinant Antibody Production HTP Recombinant Antibody Production

HTP Recombinant
Antibody Production

Antibody Production
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Our 2 week high-throughput recombinant antibody production is a leading gene-to-antibody workflow in the industry.

At Biointron, we offer the flexibility to rapidly express large quantities of antibodies at varying scales using HEK293 or CHO cells, both known for their efficiency in recombinant protein expression. We also integrate our recombinant protein service to enhance antibody expression workflows, ensuring optimized outcomes for a wide range of research and therapeutic applications. This enables us deliver high quality antibodies with low endotoxin levels (<1EU/mg) and high purity (>95% by SDS-PAGE).

Our recombinant antibody production services also include:

HTP Recombinant Antibody Production Overview

Key Benefits of Our HTP Antibody Production

2 Weeks!

  • 2 Weeks (From sequence to antibody)
  • In-house gene synthesis platform

Large Capacity

  • Automated working stations
  • Streamlined procedure

Tailored Services

  • No limitations on isotype and species
  • Multiple expression systems

Accelerate Your Pipeline with a Proven Global Leader

With over 12 years of experience in antibody discovery, expression, and optimization, Biointron has become a trusted partner to more than 2,500 biotech and pharmaceutical companies worldwide. Since 2012, we’ve delivered over 100,000 recombinant antibodies annually.

Certified to ISO 9001:2015 and supported by a global team of 600+ professionals, Biointron offers a fully integrated, high-throughput solution - from gene sequence to purified antibody - in just 2 weeks.

Our team provides the speed, quality, and scalability needed to meet the unique requirements of your program.

Custom Recombinant Antibodies Produced by Biointron

Our platform supports the production of a wide range of antibody types. The advancement of antibody discovery technologies like hybridoma, display libraries, Single B-cell sorting/sequencing, and computational modeling has led to the expression of thousands of monoclonal antibodies (mAbs). However, identifying the "best-in-class" antibody therapeutics requires rapid assessment using high-throughput (HTP) processes.

Our Step-by-Step HTP Production Process

Gene synthesis

Plasmid construction and preparation

Transient expression

Affinity purification

Stringent quality control

Fast delivery

HTP Recombinant Antibody Production Service Details

Our streamlined HTP production process ensures quality and efficiency at every step, from gene synthesis to purified antibodies.

Service Step Service Description Timeline Deliverables
Gene synthesis & cloning
  • Codon optimization and gene synthesis
  • Subcloning into an expression vector
  • Plasmid amplification and preparation
1 week
  • Expression plasmid contain GOI
  • Purified antibody (SDS-PAGE >95%, endotoxin level <1EU/mg, including SEC-HPLC report)
  • CoA report
Transient expression & purification
  • Transfection of mammalian cells
  • Transient expression and purification
1 week
  • Gene synthesis & cloning
    Timeline

    1 week

    Description
    • Codon optimization and gene synthesis
    • Subcloning into an expression vector
    • Plasmid amplification and preparation
    Deliverables
    • Expression plasmid contain GOI
    • Purified antibody (SDS-PAGE >95%, endotoxin level <1EU/mg, including SEC-HPLC report)
    • CoA report
  • Transient expression & purification
    Timeline

    1 week

    Description
    • Transfection of mammalian cells
    • Transient expression and purification
    Deliverables
    • Expression plasmid contain GOI
    • Purified antibody (SDS-PAGE >95%, endotoxin level <1EU/mg, including SEC-HPLC report)
    • CoA report

Case Study

Case 1: 818 Abs (IgG) production

Yield
Purity
Biointron finished the production of all 818 IgGs within 2 weeks.

Case 2: VHH antibody expression (Expressing data from 180 VHH-Fc)

Antibody

Expression System: CHO

SDS-PAGE Purity: > 98%

SEC-HPLC Purity: > 95%

Deliveries:> 1 mg/VHH antibody

Average: 218 mg/L

Meet RushMab™ — Delivery in as fast as 4 days!

Biointron’s RushmAb™ small-scale packages are designed to offer cost-effective, time-saving solutions to meet rush needs in as fast as 4 days!

RushMab™ - Gene

100 ug transfection grade
expression plasmids
Production Timeline: 4 days

RushMab™ - Super

1 ml supernatant,
expression plasmids
Production Timeline: 8 days

RushMab™ - Mini

≥100 ug purified antibody,
expression plasmids
Delivery in 10 days

RushMab™ - Standard

≥1 mg purified antibody,
expression plasmids
Delivery in 10 days

Team Member Spotlight

“I am proud to oversee our featured 2-week high-throughput recombinant antibody production process. Our unrivaled timing does not compromise on quality, and we have 2,500+ happy customers.”
Zhaohao Teng
Zhaohao Teng
HTP Antibody Expression Team

FAQs About HTP Recombinant Antibody Production

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