HTP Recombinant Antibody Production HTP Recombinant Antibody Production

HTP Recombinant
Antibody Production in Just 2 Weeks

  • 2 Weeks Delivery
  • Large Capacity From 100ug to 100g
  • Tailored Services
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HTP Recombinant Antibody Overview

The advancement of antibody discovery technologies like hybridoma, display libraries, Single B-cell sorting/sequencing, and computational modeling has led to the production of thousands of monoclonal antibodies (mAbs). However, identifying the "best-in-class" antibody therapeutics requires rapid assessment using high-throughput (HTP) processes.


Our 2-week high-throughput recombinant antibody production is the leading gene-to-antibody workflow in the industry. We offer the flexibility to rapidly express large amounts of antibodies at varying scales using HEK293 or CHO cells, which are known for efficient recombinant protein production. Additionally, we integrate our recombinant protein expression service to complement antibody production workflows, ensuring optimized results for diverse research and therapeutic applications.

HTP Recombinant Antibody Production Overview

Key Benefits of Our HTP Antibody Production

2 Weeks!

  • 2 Weeks (From sequence to antibody)
  • In-house gene synthesis platform

Large Capacity

  • Automated working stations
  • Streamlined procedure

Tailored Services

  • No limitations on isotype and species
  • Multiple expression systems

Our Step-by-Step HTP Production Process

Gene synthesis

Plasmid construction and preparation

Transient expression

Affinity purification

Stringent quality control

Fast delivery

HTP Recombinant Antibody Production
Service Details

Our streamlined HTP production process ensures quality and efficiency at every step, from gene synthesis to purified antibodies.

Service Step Service Description Timeline Deliverables
Gene synthesis & cloning
  • Codon optimization and gene synthesis
  • Subcloning into an expression vector
  • Plasmid amplification and preparation
1 week
  • Expression plasmid contain GOI
  • Purified antibody (SDS-PAGE >95%, endotoxin level <1EU/mg, including SEC-HPLC report)
  • CoA report
Transient expression & purification
  • Transfection of mammalian cells
  • Transient expression and purification
1 week

Integrating mammalian recombinant protein expression into our workflows, Biointron ensures the production of high-quality antibodies tailored to meet diverse research and therapeutic needs. Our cutting-edge systems streamline production while maintaining precision and scalability, making us the ideal partner for your antibody discovery projects.

Case Study

Case 1: 818 Abs (IgG) production

Yield
Purity
Biointron finished the production of all 818 IgGs within 2 weeks.

Case 2: VHH antibody expression (Expressing data from 180 VHH-Fc)

Antibody

Expression System: CHO

SDS-PAGE Purity: > 98%

SEC-HPLC Purity: > 95%

Deliveries:> 1 mg/VHH antibody

Average: 218 mg/L

“I am proud to oversee our featured 2-week high-throughput recombinant antibody production process. Our unrivaled timing does not compromise on quality, and we have 2,000+ happy customers.”
Zhaohao Teng
Zhaohao Teng
HTP Antibody Production Team

FAQs About HTP Recombinant Antibody Production

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