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Large Scale Antibody Production Large Scale Antibody Production

Large Scale Antibody Production

Antibody Production
  • Consistency
  • Large Capacity
  • 2 - 4 Weeks

Overview

Mass production of proteins is frequently influenced by factors such as cost, turnaround time, and regulatory compliance, and is subject to stringent quality control and assurance measures.1


Biointron has been dedicated to delivering high-quality products to biotechnology and pharmaceutical customers worldwide. In order to meet our customers' needs for gram-scale proteins, with over 10 years of expertise in protein production, Biointron has established a mass protein production service with validated cell culture technology, which allows rapid and large amounts of recombinant proteins production. This service offers scaled-up production of recombinant antibodies or proteins from milligram to gram levels in weeks.

Large Scale Antibody Production Overview

Highlights

Consistency

  • All products originate from a single batch
  • High QC (SDS-PAGE >95%, low endotoxin, and SEC-HPLC detection)

High Efficiency

  • Large Capacity (Hundreds of grams level)
  • Fast Turnaround (2-4 Weeks)

Flexibility

  • Transient Expression
  • Stable Cell Pool
  • Stable Cell Line
Speak to A Specialist

Flexible to Different Scales

Global Standards
Global Standards
Global Standards
Global Standards

Large Scale Antibody Production
Service Details

Service Step Service Timeline Deliverables
Gene synthesis and subcloning(Optional)
  • Codon optimization and gene synthesis
  • Subcloning into an expression vector
  • Plasmid amplification and preparation
 1 week
  • Expression plasmid contain GOI
  • Purified rAb/protein
  • CoA report
Pilot study(Optional)
  • Transfection of mammalian cells
  • Small scale pilot evaluation
  • QC analysis
Large scale production (up to 200L)
  • Maxi plasmid preparation
  • Transfection and expression
  • QC analysis
 3-4 weeks

Case Study

  • Production of 1.97 gram Antibody (Mouse IgG2a)

    Protein Marker

    M: Protein Marker

    R: Reducing

    N-R: Non-Reducing

    Detector A Channel 2 280nm

    Production of 1.97gram Antibody (Mouse lgG2a)

    Yield:288.12 mg/L,SDS-Page>95%, SEC-HPLC>99%

“If you require industrial levels of antibody production, our large-scale service is for you. You can rely on us to ensure consistency and quality across all antibodies.”
Hao Jiang
Hao Jiang
Antibody Production Team

Project Management

Our dedicated Biointron project management team assumes the crucial responsibility of being the official contact for the customer's project, ensuring its seamless development. As the primary point of communication, the account manager skillfully bridges the gap between the customer and our team of experts, guaranteeing that the project delivers the highest quality.

FAQs

  • How can a CRO help with large-scale production of therapeutic antibodies or proteins?

    Large-scale production poses unique challenges, such as maintaining product stability, scalability of the production process, and minimizing batch-to-batch variability. Additionally, ensuring the purification and characterization of the antibodies or proteins at this scale requires advanced technology and expertise. CROs specialize in providing this infrastructure, and Biointron has dedicated teams of scientists to ensure the production process runs smoothly, in addition to conducting quality control.

  • What industries and applications require large scale antibody production? 

    Large-scale antibody production is highly sought after due to the growing demand for antibodies in research, diagnostics, and therapeutics. Some of the key industries include:

    • Pharmaceuticals and Biotechnology: For the discovery and development of therapeutic antibodies to treat various diseases including cancer, autoimmune disorders, and infectious diseases.
    • Biomedical Research: For academic and commercial research laboratories to study cellular processes, identify biomarkers, validate drug targets, and understand disease mechanisms.
    • Diagnostics: For the development of diagnostic tests such as ELISA, Western blotting, flow cytometry, and immunohistochemistry, to detect specific biomolecules or diseases.
    • Vaccine Development: For producing monoclonal antibodies that can neutralize pathogens, aiding in the development of effective vaccines against various diseases.
  • How has large-scale production developed?

    Because of the clinical and commercial success of antibodies, there was a rapid expansion of global manufacturing capacity and an increase in the size of reactors to 20,000 liters. The productivity of cell cultures alone has improved over 100-fold in the last 30 years.2

Resources

References

  • National Research Council (US) Committee on Methods of Producing Monoclonal Antibodies. (1999). 5, Large-Scale Production of Monoclonal Antibodies. Monoclonal Antibody Production. Washington (DC): National Academies Press (US). Available from: https://www.ncbi.nlm.nih.gov/books/NBK100189/
  • Birch, J. R., & Racher, A. J. (2006). Antibody production. Advanced Drug Delivery Reviews, 58(5-6), 671-685. https://doi.org/10.1016/j.addr.2005.12.006
commitment

Committed to Top-Quality Products

We are deeply committed to delivering top-quality products that surpass customer expectations. With every product we offer, we promise to provide unmatched quality, reliability, and satisfaction to our valued customers. Your trust in our brand fuels our passion for continuous improvement, ensuring that we consistently deliver nothing but the best.

Services & Products

HTP Recombinant
Antibody Production
Bispecific Antibody
Production
Protein Expression In
Mammalian system
Single B Cell Screening
(Antibody Discovery)
CHO-K1 Stable
Cell line Generation
VHH Antibody
Discovery
Antibody
Humanization
Affinity
Maturation
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