Protein Expression In Mammalian

Protein Expression In Mammalian

Recombinant proteins expressed in mammalian system have a profound impact in many areas of basic and applied research, as well as the biotech/biopharma companies. For biotech/biopharma companies, they have heavily invested in the production of protein therapeutics as a relatively new and transformative approach to treating human diseases. For academic research groups, recombinant mammalian proteins will be required for functional and high-resolution structure determination. By far the greatest demand for recombinant mammalian proteins is for therapeutic development and applications.

BioIntron recombinant protein expression platform can provide high-quality protein in a flexible, cost-effective manner, in the least amount of time.

Our Advantages

Competitive Price
Competitive Price

(starting from $1000 including gene synthesis)

Fast Turnaround Time
Fast Turnaround Time

(2-3 weeks from gene synthesis to final purified protein)

Extensive Experience
Extensive Experience

(10+ years in recombinant expression with over 2000 proteins expressed, success rate of >95%)

Protein Expression In Mammalian Working Flowchart


Protein Expression In Mammalian Working Flowchart

Protein Expression In Mammalian Service Detail

Service StepService DescriptionTimelinePrice(USD)Deliverable

Gene synthesis

Codon optimization and gene synthesis

Subcloning into an expression vector

Plasmid amplification and preparation

2 to 3 weeks

Inquiry

Expression plasmid contain GOI

Purified Recombinant protein (SDS>PAGE>90%, endotoxin level <1EU/mg)

CoA report

100ml Pilot study

Transfection of mammalian cells

100ml pilot protein expression

QC analysis

Scale up production (1L-10L)

Large scale protein expression 

Affinity purification

QC analysis

3 to 4 weeks

Inquiry

Guaranteed amount purified protein (SDS>PAGE>90%, endotoxin level <1EU/mg)

Case Study: 100ml Pilot Study of a His-tag Protein in HEK293

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Lane M: Marker Lane 1: Reducing Lane 2: Non-Reducing

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SEC-HPLC Result

Expression result
Yield (mg/L)SDS-PAGE %SEC-HPLC%Endotoxin(EU/mg)Timeline
63.8>95%100%
<1EU/mg14 days
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