Protein Expression In Mammalian-Biointron

Recombinant proteins expressed in mammalian system have a profound impact in many areas of basic and applied research, as well as the biotech/biopharma companies. For biotech/biopharma companies, they have heavily invested in the production of protein therapeutics as a relatively new and transformative approach to treating human diseases. For academic research groups, recombinant mammalian proteins will be required for functional and high-resolution structure determination. By far the greatest demand for recombinant mammalian proteins is for therapeutic development and applications.

BioIntron recombinant protein expression platform can provide high-quality protein in a flexible, cost-effective manner, in the least amount of time.

Our Advantages

Competitive Price

(starting from $1000 including gene synthesis)

Fast Turnaround Time

(2-3 weeks from gene synthesis to final purified protein)

Extensive Experience

(10+ years in recombinant expression with over 2000 proteins expressed, success rate of >95%)

Protein Expression In Mammalian Working Flowchart

Protein Expression In Mammalian Service Detail

Service Step	Service Description	Timeline	Price（USD）	Deliverable
Gene synthesis	▸Codon optimization and gene synthesis ▸Subcloning into an expression vector ▸Plasmid amplification and preparation	2 to 3 weeks	Inquiry	▸Expression plasmid contain GOI ▸Purified Recombinant protein (SDS>PAGE>90%, endotoxin level <1EU/mg) ▸CoA report
100ml Pilot study	▸Transfection of mammalian cells ▸100ml pilot protein expression ▸QC analysis
Scale up production (1L-10L)	▸Large scale protein expression ▸Affinity purification ▸QC analysis	3 to 4 weeks	Inquiry	Guaranteed amount purified protein (SDS>PAGE>90%, endotoxin level <1EU/mg)

Service Step

Service Description

Timeline

Price（USD）

Deliverable

Gene synthesis

▸Codon optimization and gene synthesis

▸Subcloning into an expression vector

▸Plasmid amplification and preparation

2 to 3 weeks

Inquiry

▸Expression plasmid contain GOI

▸Purified Recombinant protein (SDS>PAGE>90%, endotoxin level <1EU/mg)

▸CoA report

100ml Pilot study

▸Transfection of mammalian cells

▸100ml pilot protein expression

▸QC analysis

Scale up production (1L-10L)

▸Large scale protein expression

▸Affinity purification

▸QC analysis

3 to 4 weeks

Inquiry

Guaranteed amount purified protein (SDS>PAGE>90%, endotoxin level <1EU/mg)

Case Study: 100ml Pilot Study of a His-tag Protein in HEK293

Lane M: Marker Lane 1: Reducing Lane 2: Non-Reducing		SEC-HPLC Result
Expression result
Yield (mg/L)	SDS-PAGE %	SEC-HPLC%	Endotoxin (EU/mg)	Timeline
63.8	>95%	100%	<1EU/mg	14 days