What materials are required to initiate an afucosylated antibody expression project?

Clients need to provide the antibody sequence.

What is the typical timeline for afucosylated antibody production?

The production process typically takes 2–4 weeks, depending on the project's complexity and specific requirements.

What quality control measures are implemented during production?

Biointron provides SDS-PAGE, SEC-HPLC, and endotoxin results by default. Additional QC measures can be included by request.

Can Biointron produce afucosylated antibodies in different formats?

Yes, Biointron can produce afucosylated antibodies in various formats, including IgG, scFv, Fab, and bispecific antibodies, tailored to meet specific research or therapeutic needs.

What deliverables will I receive upon project completion?

Clients receive purified afucosylated antibody and expression plasmids containing your gene of interest, along with a full CoA report.

Afucosylated Antibody Expression Services

Upgrade the efficacy of your antibodies with our powerful afucosylation platform in 2-4 weeks.

We use the engineered mammalian cell system CHOK1-FUT8 for afucosylated antibody expression. Integrated with our high-throughput recombinant antibody expression capability and cell line development expertise, afucosylated antibodies can be rapidly produced to meet your research needs.

We will provide you with high quality antibodies with low endotoxin levels (<1EU/mg) and high purity (>95% by SDS-PAGE).

Our recombinant antibody production services also include:

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Afucosylated Antibody Expression Overview

Antibodies with high purity and low endotoxin levels!

Afucosylation has been shown to significantly enhance antibody-dependent cell-mediated cytotoxicity (ADCC). This antibody modification has unlocked new possibilities for improving the efficacy of immunotherapies involving certain types of immune effector cells.

At Biointron, a Fut8-knockout CHO cell line is applied for the expression of afucosylated antibodies. Fut8 is an enzyme that catalyzes the formation of a-1,6 fucosyl glycosidic bonds, and thus the addition of fucose to asparagine-linked N-acetylglucosamine moieties.

Biointron's fucose-free host cell CHOK1BN-Fut8KO can be used for industrial production after antibody characterization and functional verification. Compared to the unmodified CHOK1BN cell line, the antibody produced by the modified cell line not only achieves afucosylation but also maintains consistency in terms of expression quantity and quality.

Key Benefits of Our Fut8KO Afucosylated Antibody Production

Advanced Gene-Editing Technology

Our technology enables efficient Fut8 gene knockouts
We guarantee the fucose knockout in host cells

High Yield and Quality

Extensive experience in CHO stable cell line development for over 10 years
Flexible for large scale afucosylated antibody production
High purity & low endotoxin level, optional LC-MS analysis can be performed to verify the absence of fucosylation.

Customized Sequence, Customized Expression

Simply provide your customized sequences and we will express your antibodies
Fast antibody products delivery

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Rapidly Express Your Afucosylated Antibodies

Our platform supports the production of a wide range of antibody types. At Biointron, we focus exclusively on antibody services, earning the trust of more than 2,500 biotech and pharmaceutical companies worldwide through our discovery, expression, and optimization solutions.

Certified to ISO 9001:2015 and supported by a global team of over 600 professionals, Biointron offers a fully integrated, high-throughput workflow, from gene sequence to purified antibody, in just 2 weeks.

Our team provides the speed, quality, and scalability needed to meet the unique requirements of your program.

Case Study

Case 1:

No.	Host cell	INN
001	CHOK1BN	Rituximab
002	CHOK1BN-Fut8KO	Rituximab

Expression results (SDS-PAGE & analytics SEC-HPLC)

001

002

Reduced mass analysis

The reduced mass matched the theoretical molecular weight of 001, the main N-glycosylation type on the heavy chain was G0, G1, and N-glycosylation type contain fucose.

The reduced mass matched the theoretical molecular weight of 002, the main N-glycosylation type on the heavy chain was G0, G1, and no N-glycosylation type contain fucose was found.

PBMC killing assay (Raji)

The results showed both antibodies demonstrated target cell killing activity, indicating their strong potential for immune-mediated cytotoxicity.

Case 2:

MW: 52/48/23kDa (123kDa)

This case highlights the production of a bispecific antibody using CHOK1BN-Fut8KO cells. After one-step Protein A purification, SDS-PAGE and analytical SEC-HPLC revealed the presence of high molecular weight impurities. A second round of purification was performed to improve purity. Reduced MS analysis confirmed that the antibody was afucosylated, with no N-glycosylation at the Fc region.

1^st purification

2^nd purification

The reduced mass matched the theoretical molecular weight of the light chain, and there is no N-glycosylation site.

The reduced mass matched the theoretical molecular weight of the heavy chain, and no N-glycosylation type contain fucose was found.

Team Member Spotlight

“The newest offering in our service catalog, Biointron’s CHOK1-FUT8 afucosylated antibody expression, is an exciting addition to our capabilities. Heavily researched, afucosylation will improve your antibody efficacy, and my seasoned team of scientists will make sure the end results go above and beyond expectations.”

Xinyi Zhang

Antibody Production Team

FAQs About Afucosylated Antibody Production

What materials are required to initiate an afucosylated antibody expression project?

Clients need to provide the antibody sequence.
2.What expression systems are used for afucosylated antibody production?

Biointron uses the engineered mammalian cell system CHOK1-FUT8 for afucosylated antibody expression.
What is the typical timeline for afucosylated antibody production?

The production process typically takes 2–4 weeks, depending on the project's complexity and specific requirements.
What quality control measures are implemented during production?

Biointron provides SDS-PAGE, SEC-HPLC, and endotoxin results by default. Additional QC measures can be included by request.
Can Biointron produce afucosylated antibodies in different formats?

Yes, Biointron can produce afucosylated antibodies in various formats, including IgG, scFv, Fab, and bispecific antibodies, tailored to meet specific research or therapeutic needs.
What deliverables will I receive upon project completion?

Clients receive purified afucosylated antibody and expression plasmids containing your gene of interest, along with a full CoA report.

Resources

Biointron_Full Services.pdf

Committed to Top-Quality Products

At Biointron, we are deeply committed to delivering top-quality products that surpass customer expectations. With every product we offer, we promise to provide unmatched quality, reliability, and satisfaction to our valued customers. Your trust in our brand fuels our passion for continuous improvement, ensuring that we consistently deliver nothing but the best.

CHOK1-FUT8 Afucosylated
Antibody Expression

Upgrade the efficacy of your antibodies with our powerful afucosylation platform in 2-4 weeks.