Bispecific Antibody Production

Bispecific Antibody Production

With the current biotherapeutic market dominated by antibody molecules, bispecific antibodies represent a key component of the next-generation of antibody therapy. Bispecific antibodies can target two different antigens at the same time, such as simultaneously binding tumor cell receptors and recruiting cytotoxic immune cells.

BioIntron has expressed thousands bispecific antibodies in 2020 with extensive experience in bispecific antibody production. BioIntron can perform any format bispecific antibody production requested by our client.  

Our Advantages

Competitive Price
Competitive Price

(starting from $1,000 including gene synthesis)

Extensive Experience And Strong Expertise
Extensive Experience And Strong Expertise

(produce thousands BsAb production per year, >20K mAb expressions per year. Any format BsAb can be customized)

Fast Turnaround Time
Fast Turnaround Time

(only 3 weeks from gene sequence to purified BsAb)

Bispecific Antibody Production Working Flowchart


Bispecific Antibody Production Working Flowchart

Bispecific Antibody Production Service Detail

Service StepService DescriptionTimelinePrice(USD)Deliverable

Gene synthesis and cloning

Codon optimization and gene synthesis

Subcloning into an expression vector

Plasmid amplification and preparation

1-2weeks

Inquiry

CoA report

Expression plasmid contain GOI

Purified antibody (SDS-PAGE>95%, endotoxin level <1EU/mg, including SEC-HPLC detection)

Transient expression & purification

Co-Transfection of mammalian cells

Transient expression and purification

QC analysis

1week

Case Study: 100ml pilot study for bispecific antibody production

One step purification


Lane M: Marker Lane 1: Non-Reducing Lane 2:Reducing

Lane M: Marker Lane 1: Non-Reducing Lane 2:Reducing


SEC-HPLC Detection Result

SEC-HPLC Result

After 2nd purification


Lane M: Marker Lane 1: Non-Reducing Lane 2:Reducing

Lane M: Marker Lane 1: Non-Reducing Lane 2:Reducing


SEC-HPLC Detection Result

SEC-HPLC Result

Expression result
Yield (mg/L)SDS-PAGE %SEC-HPLC%Endotoxin(EU/mg)Timeline
199mg/L>95%98.14%<1EU/mg20 days
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