CHO-K1 Stable Cell line Generation

CHO-K1 Stable Cell line Generation

Cell line development plays a vital role in drug development. It is the bridge connecting drug discovery and development. Therefore, It is utmost importance to develop more efficient production cell lines for recombinant proteins/antibody that meet industrial requirements. A reliable cell line development service can always save your valuable time and lower your final cost.

BioIntron can provide stable cell line development service for bioproduction purpose. Our stable cell line service, based on ECACC licensed CHO-K1 cell and strongest expertise, aims to provide high-yield guaranteed and speedy timeline to meet our client’s downstream CMC development application.

Our Advantages

High-yield Guaranteed
High-yield Guaranteed

(>5gram/L for antibody)

Extensive Experience And Strong  Expertise
Extensive Experience And Strong Expertise

(10+ years in stable cell line development with over 100 high-yield cell lines delivered) 

One-time Sublicense Fee For Multiple Products
One-time Sublicense Fee For Multiple Products

(The licenses is perpetual and irrevocable)

CHO-K1 Stable Cell line Generation Working Flowchart


CHO-K1 Stable Cell line Generation Working Flowchart

CHO-K1 Stable Cell Line Development Service Detail

Service StepService DescriptionTimelinePrice(USD)Deliverable
Option 1: cell line development

Gene synthesis and transient expression

Codon optimization and gene synthesis

Subcloning into expression vector

Transient expression

2-3 weeks

Inquiry

Gene optimized for the required expression system

Gene cloned in expression plasmid

Stable pool generation

Stable transfection

Stable Pool selection and amplification

8-9 weeks
Single clone selection and screening

Subcloning of the top producing clones

Characterization of clones

8-9 weeks

50mL of the supernatant from stable pool

Detailed report

Primary Cell Bank Generation

Primary Cell Bank (PCB) preparation

PCB Stability Study

~3 months

Primary cell bank -tested for Mycoplasma

Certificate of Analysis

Detailed report

CHO-K1 cell report

Detail CHO-K1 cell line report and document

N/A

Detail CHO-K1 cell line report and document

Option 2: CHO-K1 cell sublicense service
CHO-K1 sublicense

Detail CHO-K1 cell line report and document

CHO-K1 sublicense agreement

3-5 daysInquiry

2 vial CHO-K1 suspension cell

Detail CHO-K1 cell line report and document

CHO-K1 sublicense agreement

Case Study: Chickenpox Vaccine (Tag free) Stable Cell Line Development

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Monoclonality Proving


Monoclonality Proving


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