CHO-K1 Stable Cell line Generation-Biointron

Cell line development plays a vital role in drug development. It is the bridge connecting drug discovery and development. Therefore, It is utmost importance to develop more efficient production cell lines for recombinant proteins/antibody that meet industrial requirements. A reliable cell line development service can always save your valuable time and lower your final cost.

BioIntron can provide stable cell line development service for bioproduction purpose. Our stable cell line service, based on ECACC licensed CHO-K1 cell and strongest expertise, aims to provide high-yield guaranteed and speedy timeline to meet our client’s downstream CMC development application.

Our Advantages

High-yield Guaranteed

(>5gram/L for antibody)

Extensive Experience And Strong Expertise

(10+ years in stable cell line development with over 100 high-yield cell lines delivered)

One-time Sublicense Fee For Multiple Products

(The licenses is perpetual and irrevocable)

CHO-K1 Stable Cell line Generation Working Flowchart

CHO-K1 Stable Cell Line Development Service Detail

Service Step	Service Description	Timeline	Price（USD）	Deliverable
Option 1: cell line development
Gene synthesis and transient expression	▸Codon optimization and gene synthesis ▸Subcloning into expression vector ▸Transient expression	2-3 weeks	Inquiry	▸Gene optimized for the required expression system ▸Gene cloned in expression plasmid
Stable pool generation	▸Stable transfection ▸Stable Pool selection and amplification	8-9 weeks
Single clone selection and screening	▸Subcloning of the top producing clones ▸Characterization of clones	8-9 weeks		▸50mL of the supernatant from stable pool ▸Detailed report
Primary Cell Bank Generation	▸Primary Cell Bank (PCB) preparation ▸PCB Stability Study	~3 months		▸Primary cell bank -tested for Mycoplasma ▸Certificate of Analysis ▸Detailed report
CHO-K1 cell report	▸Detail CHO-K1 cell line report and document	N/A		▸Detail CHO-K1 cell line report and document
Option 2: CHO-K1 cell sublicense service
CHO-K1 sublicense	▸Detail CHO-K1 cell line report and document ▸CHO-K1 sublicense agreement	3-5 days	Inquiry	▸2 vial CHO-K1 suspension cell ▸Detail CHO-K1 cell line report and document ▸CHO-K1 sublicense agreement

Case Study: Chickenpox Vaccine (Tag free) Stable Cell Line Development

Monoclonality Proving