What scales of antibody production does Biointron offer?

Biointron provides scalable antibody production services, ranging from 100 µg to 100 g, to accommodate various research, diagnostic, and therapeutic needs.

What expression systems are used for large-scale production?

Biointron utilizes mammalian cell systems, such as CHO-K1/HEK293, or bacterial systems such as E. coli, for antibody expression, ensuring high yield and quality.

What is the typical timeline for large-scale antibody production?

The production process can be as fast as 2 weeks, depending on the requested scale. For gram-level production, we can deliver as fast as 4 weeks.

What quality control measures are implemented during production?

Biointron provides SDS-PAGE, SEC-HPLC, and endotoxin results by default. Additional QC measures can be included by request.

Large-Scale Antibody Production Services

Gram-level antibody production in 2-4 weeks high-throughput recombinant antibody expression.

Mass production of proteins is frequently influenced by factors such as cost, turnaround time, and regulatory compliance, and is subject to stringent quality control and assurance measures.

Biointron has been dedicated to delivering high-quality products to biotechnology and pharmaceutical customers worldwide. To meet the growing demand for gram-scale proteins, and with over 10 years of expertise in protein production, Biointron has established a large-scale protein expression service using validated cell culture technology. This enables the rapid and efficient expression of large quantities of recombinant proteins. This service supports scaled-up production of recombinant antibodies or proteins from milligram to gram levels within weeks.

Our recombinant antibody production services also include:

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Large Scale Antibody Expression Overview

Advantages of our Large-Scale Antibody Production

Consistency

All Products Originate From A Single Batch
Rigorous QC (SDS-PAGE >95%, low endotoxin, and SEC-HPLC detection)

High Efficiency

Large Capacity (Hundreds of grams level)
Fast Turnaround (2-4 Weeks)

Flexibility

Transient Expression
Stable Cell Pool
Stable Cell Line

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Efficiency in Large-Scale Antibody Production

Biointron utilizes mammalian cell systems such as CHO-K1 and HEK293, as well as bacterial systems like as E. coli, for antibody expression, ensuring high yield and consistent quality. With over 12 years of experience in antibody discovery, expression, and optimization, we are a trusted partner to over 3,000 biotech and pharmaceutical companies worldwide. Since 2012, we’ve delivered over 100,000 recombinant antibodies annually.

Certified to ISO 9001:2015 and supported by a global team of 600+ professionals, Biointron offers a fully integrated, high-throughput solution from gene sequence to purified antibody in just 2 weeks.

A Dedicated Team for Antibody Expression

Our dedicated Biointron project management team serves as primary contact for your project, ensuring smooth execution from start to finish. Your account manager will coordinate closely with our team of experts to maintain clear communiaton and deliver precisely what you need.

At Biointron, we offer the flexibility to rapidly express large quantities of antibodies at various scales. We support a wide range of antibody formats, including IgG, scFv, Fab, and bispecific antibodies, customized to meet specific research or therapeutic requirements. All antibodies are delivered with low endotoxin levels (<1EU/mg) and high purity (>95% by SDS-PAGE).

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Large Scale Antibody Expression Service Details

Service Step	Service	Timeline	Deliverables
Gene synthesis and subcloning	Codon optimization and gene synthesis Subcloning into an expression vector Plasmid amplification and preparation	2 weeks	Expression plasmid contain GOI Purified rAb/protein CoA report
Pilot study(Optional)	Transfection of mammalian cells Small scale pilot evaluation QC analysis	2 weeks
Large scale production (up to 200L)	Maxi plasmid preparation Transfection and expression QC analysis	Transient Expression: 3-4 weeks Stable Expression: 2-3 months

Gene synthesis and subcloning
Timeline

2 weeks for gene synthesis and subcloning & pilot study

Description
- Codon optimization and gene synthesis
- Subcloning into an expression vector
- Plasmid amplification and preparation
Pilot study (Optional)
Timeline

2 weeks for gene synthesis and subcloning & pilot study

Description
- Transfection of mammalian cells
- Small scale pilot evaluation
- QC analysis
Large scale production (up to 200L)
Timeline

3-4 weeks

Description
- Maxi plasmid preparation
- Transfection and expression
- QC analysis
Deliverables
- Expression plasmid contain GOI
- Purified rAb/protein
- CoA report

Case Study

Production of 1.97 gram Antibody (Mouse IgG2a)

M: Protein Marker

R: Reducing

N-R: Non-Reducing

Production of 1.97gram Antibody (Mouse lgG2a)

Yield:288.12 mg/L,SDS-Page>95%, SEC-HPLC>99%

Team Member Spotlight

“If you require industrial levels of antibody production, our large-scale service is for you. You can rely on us to ensure consistency and quality across all antibodies.”

Qianjin Cheng

Antibody Expression Team

Project Management

Our dedicated Biointron project management team assumes the crucial responsibility of being the official contact for the customer's project, ensuring its seamless development. As the primary point of communication, the account manager skillfully bridges the gap between the customer and our team of experts, guaranteeing that the project delivers the highest quality.

Large-Scale Antibody Production FAQs

What scales of antibody production does Biointron offer?

Biointron provides scalable antibody production services, ranging from 100 µg to 100 g, to accommodate various research, diagnostic, and therapeutic needs.
What expression systems are used for large-scale production?

Biointron utilizes mammalian cell systems, such as CHO-K1/HEK293, or bacterial systems such as E. coli, for antibody expression, ensuring high yield and quality.
What is the typical timeline for large-scale antibody production?

The production process can be as fast as 2 weeks, depending on the requested scale. For gram-level production, we can deliver as fast as 4 weeks.
What quality control measures are implemented during production?

Biointron provides SDS-PAGE, SEC-HPLC, and endotoxin results by default. Additional QC measures can be included by request.
Can Biointron produce antibodies in different formats?

Yes, Biointron can produce antibodies in various formats, including IgG, scFv, Fab, and bispecific antibodies, tailored to meet specific research or therapeutic needs.

Resources

Biointron_Full Services.pdf

Committed to Top-Quality Products

We are deeply committed to delivering top-quality products that surpass customer expectations. With every product we offer, we promise to provide unmatched quality, reliability, and satisfaction to our valued customers. Your trust in our brand fuels our passion for continuous improvement, ensuring that we consistently deliver nothing but the best.

Large Scale Antibody Expression

Gram-level antibody production in 2-4 weeks high-throughput recombinant antibody expression.

Advantages of our Large-Scale Antibody Production

Consistency

High Efficiency

Flexibility

Flexible to Different Scales

Efficiency in Large-Scale Antibody Production

A Dedicated Team for Antibody Expression

Large Scale Antibody Expression Service Details

Case Study

Production of 1.97 gram Antibody (Mouse IgG2a)

Team Member Spotlight

Qianjin Cheng

Antibody Expression Team

Project Management

Large-Scale Antibody Production FAQs

Resources

Committed to Top-Quality Products

Services & Products