Authors: Ying Liu, Shasha Jiao, Yunyun Chang, Xinying Lu, Pengyan Liu, Ying Zhao, Changchun Zha, Lirong Shen, Yirong Guo, Guonian Zhu
Journal: Food and Agricultural Immunology
DOI: https://doi.org/10.1080/09540105.2020.1797640
Products & Services: Subtype-specific (κ and IgG) degenerated primers of VL and VH; Upstream and downstream primers were designed based on not only the sequences of variable regions of PY-C7-mAb but also the homologous sequences of the expression vector plasmids; HEK293(F) cells were suspension cultured and passaged with limited component medium (CD-293 medium, Biointron, Cat#BI293001).
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