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Single B Cell Screening

Biointron 2024-01-26 Read time: 1 min

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Single B cell screening is a powerful technique for isolating and generating antigen-specific monoclonal antibodies (mAbs). This method, often integrated with high-throughput platforms, allows for efficient detection of antibody secretion and rapid identification of lead clones, thereby accelerating antibody discovery, vaccine design, and the development of targeted therapies. Single B cells are advantageous for its simplicity, as it requires only a small number of cells while maintaining a high efficiency in rapidly obtaining specific mAbs.1


The overall process is as follows:

  1. Immunization: 1-2 months with host species with a specific antigen of interest.

  2. Plasma B cell isolation: Collect antibody-secreting cells from spleen and bone marrow.

  3. Single B cell screening: Protein binding and/or cell-based binding is used to detect and sort positive micro-droplets.

  4. Single-cell sequencing.

  5. High-throughput expression and validation.2


Biointron's high-throughput single B cell screening platform can screen 2*10^6 plasma B-cells from immunized animals. Antigen specific antibody-secreting cells can be isolated, allowing for the detection of potentially rare antibodies and drugs. 


References:

  1. Tiller, T. (2011). Single B cell antibody technologies. New Biotechnology, 28(5), 453-457. https://doi.org/10.1016/j.nbt.2011.03.014

  2. Pedrioli, A., & Oxenius, A. (2021). Single B Cell Technologies for monoclonal antibody discovery. Trends in Immunology, 42(12), 1143–1158. https://doi:10.1016/j.it.2021.10.008

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