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Anti-Mouse Antibodies

Biointron 2024-09-23 Read time: 4 mins

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Anti-mouse antibodies are important tools in preclinical studies using mouse models, which remain the gold standard for understanding biological mechanisms and evaluating therapeutic targets. These antibodies are designed for specificity and effectiveness in murine systems, ensuring that researchers can streamline their workflows while maintaining the integrity of their experimental designs. Biointron’s Abinvivo brand provides a series of research-grade in vivo reagents tailored for this purpose. 

Key Features of Anti-Mouse Antibodies for Research

Anti-mouse antibodies typically target key immune checkpoints and cell surface markers, essential for studying immune modulation, cancer biology, and autoimmune diseases. A product example is Anti-Mouse PD-1 (RMP1-14), a reagent for blocking PD-1/PD-L1 signaling, commonly used in cancer immunotherapy research. 

Product Overview: Anti-Mouse PD-1 (RMP1-14)

  • Reactivities: Mouse 

  • Target: PD-1 (Programmed cell death protein 1), a key player in immune checkpoint regulation. PD-1 inhibits T-cell activity, and blocking this pathway can restore immune response against tumors. 

  • Isotype: Rat IgG2A-Kappa 

  • Recommended Controls: Anti-HEL Rat IgG2A-Kappa Isotype Control 

  • Applications: The primary application for this antibody is the in vivo blocking of PD-1/PD-L1 signaling, which is used to study immune evasion in tumors. PD-1 pathway blockade mimics the therapeutic mechanism of immune checkpoint inhibitors used in oncology. 

  • Formulation: PBS buffer at pH 7.4, free from stabilizers and preservatives, ensuring compatibility with sensitive in vivo systems. 

This antibody is produced using a CHO (Chinese Hamster Ovary) expression system and purified via REX, a technology that ensures the antibody meets high standards of purity (>95% based on SDS-PAGE). These qualities make it suitable for rigorous in vivo experiments, where variables like endotoxin levels (<1 EU/mg) and sterility are critical to prevent unwanted immune responses. 

Storage conditions require maintaining the antibody at 4°C, avoiding freeze-thaw cycles, to preserve its functional integrity for repeated use. 

Anti-Mouse Antibodies Targeting Immune Checkpoints

Beyond PD-1, other key immune regulators are available as anti-mouse antibodies, designed to study the immune landscape of murine models: 

  • Anti-Mouse PD-L1: This antibody targets PD-L1, the ligand for PD-1, and is equally important in blocking immune suppression. 

  • Anti-Mouse CTLA-4: Targeting CTLA-4 is another critical checkpoint strategy, used to understand T-cell activation and immune modulation in both cancer and autoimmune disease models. 

  • Anti-Mouse CD4, CD8A: These antibodies are essential for studying T-cell subsets, enabling researchers to track helper (CD4) and cytotoxic (CD8) T-cell populations in various immune responses. 

These antibodies allow researchers to study immune pathways, offering insights into how the body regulates immunity and how therapeutic interventions can tip the balance toward beneficial outcomes. 

Related: Immune Checkpoint Inhibitors and Antibody Therapeutics: A Paradigm Shift in Cancer Treatment

Customizing Anti-Mouse Antibody Applications

The utility of these antibodies extends beyond blocking pathways. Many of them, including Anti-Mouse CD3E, which targets the epsilon chain of CD3 in T-cell receptor complexes, are critical for understanding T-cell activation. Similarly, Anti-NK1.1 is used to study natural killer (NK) cells, while Anti-LY6G serves to investigate neutrophil populations. 

Due to their versatile nature, these reagents can be applied across different experiments. For example, in flow cytometry, they allow for the precise sorting of immune cell populations, while in immunohistochemistry (IHC) and functional assays, they help study tissue-specific immune responses.  

Each antibody undergoes rigorous testing to ensure purity, sterility, and low endotoxin levels, as these are critical for ensuring that experimental data are not skewed by off-target effects or contamination. With these quality standards, researchers can confidently move forward with in vivo models that closely mirror human diseases.  

Biointron’s catalog products for in vivo research can be found at Abinvivo, where we have a wide range of Benchmark Positive Antibodies, Isotype Negative Antibodies, Anti-Mouse Antibodies, Bispecific Antibodies, and Antibody-Drug Conjugates. Contact us to find out more at info@biointron.com or +86 400-828-8830 / +1(732)790-8340.

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