Resources Blog Using Recombinant Antibody Production to Create Antibodies

Using Recombinant Antibody Production to Create Antibodies

Biointron 2024-02-27 Read time: 3 mins
Image credit: DOI: 10.1007/s00114-002-0400-5

Recombinant antibody production is a revolutionary technology that has significantly impacted biomedical research and therapeutic applications. Unlike traditional antibody generation methods that rely on the immune response in animals, recombinant techniques involve the use of genetic engineering to produce antibodies with high specificity and reproducibility.  

Antibodies, or immunoglobulins, are pivotal to the immune response, recognizing and neutralizing pathogens such as bacteria and viruses. Traditionally, antibodies were produced by immunizing animals and extracting the serum. However, recombinant antibody production bypasses the need for animal immunization, using instead genetically modified cells to produce desired antibodies. 

Recombinant Antibody Production Process 

1. Gene Isolation and Cloning 

The process begins with isolating the gene that encodes the antibody of interest. This gene is then cloned into a vector, a DNA molecule used to transport genetic material into a host cell. 

2. Host Cell Selection and Modification 

Common host cells, including bacteria (E. coli), yeast, and mammalian cells, are genetically modified to incorporate the antibody gene. This modification enables the cells to produce the antibody. 

3. Cultivation and Expression 

The modified host cells are cultivated in bioreactors, where conditions are optimized for cell growth and antibody expression. This stage involves careful control of temperature, pH, and nutrient supply. 

4. Purification and Verification 

Following expression, the antibodies are purified from the culture medium. Techniques such as affinity chromatography ensure the isolation of high-purity antibodies. The specificity and activity of the antibodies are then verified through various assays. 

Advantages of Recombinant Antibody Production 

Recombinant antibodies offer several advantages over traditional methods, including higher specificity, the ability to produce antibodies against a wide range of antigens, and improved reproducibility. They can also be engineered to enhance their therapeutic efficacy. Recombinant antibodies have diverse applications, from therapeutic agents in the treatment of cancer, autoimmune diseases, and infections to diagnostic tools in medical research and clinical diagnostics.

Subscribe to our Blog

Recent Blog

Computational antibody methods schematic. DOI: 10.1093/bib/bbz095The development of therapeutic antibodies has been significantly enhanced by advancements in computational methods and artificial intelligence (AI). These technologies have streamlined the antibody discovery process, improving the abil

May 27, 2024

Welcometo Antibody Basics by Biointron,Part 8. In this episode, we’ll talk about therapeutics targeting cancer.What is cancer immunotherapy?Cancer immunotherapy leverages the body's immune system to fight cancer more selectively and effectively than traditional methods such as chemotherapy

May 24, 2024

The generation of an immune response to a vaccine. DOI: 10.1038/s41577-020-00479-7Vaccination is one of the most effective tools in preventing infectious diseases. At its core, the success of a vaccine hinges on its ability to induce a robust and lasting antibody response against a specific pathogen

May 22, 2024

Innovation orientation and goals for transforming CAR-T cell engineering. DOI: 10.1186/s13045-020-00910-5Chimeric antigen receptor (CAR)-T cell therapy is a revolutionary cancer treatment in which engineered CARs redirect lymphocytes, typically T cells, to recognize and destroy cells expressing a sp

May 20, 2024

Our website uses cookies to improve your experience. Read our Privacy Policy to find out more.